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Randy Schekman (Berkeley) Part 1: Studying Protein Secretion in Yeast

http://www.ibioseminars.org/lectures/bio-mechanisms/randy-schekman.html Protein secretion is executed by a cellular pathway involving the delivery of membrane and soluble secretory proteins in vesicles that capture newly-synthesized proteins assembled in the endoplasmic reticulum (ER) and sorted in the Golgi apparatus. Vesicles fuse with the plasma membrane resulting in the discharge of soluble molecules to the cell exterior and integration of vesicle membrane proteins and lipids in the cell surface. Baker's yeast cells grow by vesicle fusion and secretion at the tip of the daughter bud. A genetic dissection of this process was performed with temperature sensitive conditional mutants blocked at one of several stations in the secretory pathway. See more at http://www.ibioseminars.org
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