Organic chemistry: Amino acid synthesis--Gabriel synthesis; Strecker synthesis. Edman degradation. Polypeptide synthesis--Cbz (carbobenzoxy) and Boc (tert-butoxycarbonyl) amino-protecting groups; protection of the carboxy terminus via ester formation; DCC (dicyclohexylcarbodiimide) carboxy-activating reagent. An example of calculating pI and charge at a specific pH for a long polypeptide This is a recording of a tutoring session, posted with the students' permission. These videos are offered on a "pay-what-you-like" basis. You can pay for the use of the videos at my website: http://www.freelance-teacher.com/videos.htm For a printable document containing the amino acid table discussed in this video series, go to my website. For a list of all the available video series, arranged in suggested viewing order, go to my website. For a playlist containing all the videos in this series, click here: http://www.youtube.com/watch?v=gmKflNQq3LM&feature=PlayList&p=FF5C66F40E5BFE7E&index=0&playnext=1 (1) Determining charge of an amino acid at a particular pH. Acidic vs. basic side chains. Gabriel synthesis of amino acids (2) Gabriel synthesis continued (3) Continued (4) Continued. Strecker synthesis of amino acids (5) Continued (6) Continued (7) Edman degradation (8) Continued (9) Continued. Polypeptide synthesis; Cbz (carbobenzoxy) amino-protecting group (10) Cbz continued; Boc (tert-butoxycarbonyl) amino-protecting group (11) Polypeptide synthesis continued; protection of the carboxy terminus via ester formation; DCC (dicyclohexylcarbodiimide) carboxy-activating reagent (12) DCC continued (13) An example of calculating pI and charge at a specific pH for a long polypeptide (14) A polypeptide synthesis problem, illustrating the use of amino- and carboxy-protecting groups (15) Continued
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