Check us out at http://www.tutorvista.com//videos Budding in hydra has received the attention of only a few investigators. Our principal information as to the origin of the bud is derived from Lang's' account. He describes the bud as beginning by an increase in volume and division of the interstitial cells. After the ectoderm becomes thickened, the mesoglea dis appears and the cells pass from the ectoderm into the endoderm. This process continues until the ectoderm becomes reduced to its normal thickness. The mesoglea then reforms and a cavity ap pears in the thickened endoderm, which becomes the enteron of the new individual. The main object of the present paper will be to give a brief account of the origin and development of the buds in hydra, more especially their manner of growth and what cells contribute most to their rapid formation. The species studied, Hydra viridis and Hydra sp.? (Brauer) differ somewhat from Lang's account. The mesoglea does not disappear and the ectodermal cells do not pass into the endoderm. The bud, however, begins by an increase in volume and division of the interstitial cells. After they have increased once or twice in volume, as shown in Fig. i, there is a slight outbulging of the ectoderm, which is scarcely perceptible. represents the condition of the interstitial, ectodermal and endodermal cells in the origin of the bud as they appear more highly magnified. A few mitotic figures are visible. No amitotic divisions were observed. The endodermal cells contain numerous food particles, which may pass intact through the mesoglea into the ectoderm. The cells directly concerned in the formation of the bud are well supplied with food, while the remaining cells of the parent hydra show a scarcity. Many of the endodermal cells in the distal half of the hydra have a glandular appearance and are most active
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