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Polymerase Chain Reaction (Amrita University)

Polymerase chain reaction, better known as PCR, is one of the technologies that not only made a tremendous impact on the scientific community, but also affected many aspects of our everyday lives. More than 30 years ago, the introduction of recombinant DNA technology as a tool for the biological sciences revolutionized the study of life. Molecular cloning allowed the study of individual genes of living organisms; however this technique was dependent on obtaining a relatively large quantity of pure DNA. This depended on the replication of the DNA of plasmids or other vectors during cell division of microorganisms.
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