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Motility Determination in Bacteria (Amrita University)

There are three ways to determine whether a bacterium is motile (able to swim).First, you can observe live bacteria directly using hanging drop or wet mount preparations and special optics, such as darkfield or phase contrast. Second, you can stain flagella using special stains. Third,you can detect motility by growing cultures in specialized media that show whether bacteria have swum away from the initial inoculation.
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