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Preparation of Equilibrated Phenol (Amrita University)

Visit : application of molecular biology techniques to the analysis of complex genomes depends on the ability to prepare pure, high molecular wt. DNA. For applications requiring a higher pH, such as the isolation of large intact genomic DNA, addition of the Equilibrated phenol is recommended.Equilibrated phenol is one such reagent used in nucleic acid purification procedures and dissolution of protein.The chemical formula of phenol is C6H5OH and as a solid it is a white crystalline compound. Its melting point is 41° C and its boiling point is 182° C. Phenol has a sweet odour that is detectable at 0.06 PPM, which enables it to be used as an air freshener. However, the chemical is highly corrosive and moderately toxic. Therefore it is also used as a powerful disinfectant and a germicide.
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