Watch more Science Basics videos: http://www.howcast.com/guides/70-Science-Basics Subscribe to Howcast's YouTube Channel - http://howc.st/uLaHRS Follow these steps to prepare a standard EDTA solution of dihydrate ethylenediaminetetraacetic acid and water. Howcast uploads the highest quality how-to videos daily! Be sure to check out our playlists for guides that interest you: http://howc.st/ytmainplaylists Subscribe to Howcast's other YouTube Channels: Howcast Health Channel - http://howc.st/HOE3aY Howcast Video Games Channel - http://howc.st/tYKKrk Howcast Tech Channel - http://howc.st/rx9FwR Howcast Food Channel - http://howc.st/umBoJX Howcast Arts & Recreation Channel - http://howc.st/vmB86i Howcast Sports & Fitness Channel - http://howc.st/vKjUjm Howcast Personal Care & Style Channel - http://howc.st/vbbNt3 Howcast empowers people with engaging, useful how-to information wherever, whenever they need to know how. Emphasizing high-quality instructional videos, Howcast brings you experts who provide accurate information in easy-to-follow tutorials on everything from makeup, hairstyling, nail art design, and soccer to parkour, skateboarding, dancing, kissing, and much, much more. Step 1: Dry and cool Measure 4 grams of purified dihydrate ethylenediaminetetraacetic acid on the balance and dry at 80 degrees Celsius for one hour before letting it cool to room temperature in the desiccator. Tip The salt will contain about 0.3 percent moisture after drying and cooling. Make a note to correct the salt's weight in your calculation of the molarity of the finished solution. Step 2: Measure Weigh 3.8 grams and transfer it into a volumetric flask using a powder funnel. Step 3: Add water Rinse the funnel by pouring a small amount of distilled or deionized water through it before removing the salt. Then add enough extra purified water to make 700 milliliters. Use a dry, uncontaminated container with a non-glass stopper. Step 4: Mix Swirl the solution until all of the powder has dissolved. Tip Be patient -- it may take up to 15 minutes for the solution to be completely dissolved. Step 5: Add water and mix Add more purified water until it reaches the 1-liter mark, and mix well. Step 6: Calculate molarity Calculate the molarity, or molar concentration, of the solution. The result should be at or very close to 0.01 M. Step 7: Finish Store the solution with a stopper. If you plan to use it for titration, it's good practice to conduct the experiment while the solution is still fresh. Did You Know? EDTA was first used as a medical treatment for heavy metal poisoning in the 1940s, and is still FDA-approved for that purpose.
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